Acetic Acid - ANSWER Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the
... [Show More] nucleoproteins and fix the nuclei.
Acetic Acid - ANSWER Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives.
Formaldehyde - ANSWER Non coagulant and additive fixative that reacts with amino groups that link protein chains together.
Formaldehyde - ANSWER Greatest binding occurs between pH 7.5-8.
Formaldehyde - ANSWER Preserves lipids but does not make them insoluble and can be lost during subsequent processing.
Formaldehyde - ANSWER Does not preserve carbohydrates but stabilizes and fixes the proteins to trap glycogen.
Formaldehyde - ANSWER Penetrates tissue quickly, fixes slowly and has the least shrinking effect any fixative.
Formaldehyde - ANSWER Beside ethanol and acetone, this hardens tissue more than any other fixative.
Microcrystalline dark brown pigment - ANSWER Formed when acidic (<6.0 pH) solutions of formaldehyde act on bloody tissue.
Black acid hematin / formalin pigment - ANSWER When formalin pH drops below 6.0 this pigment forms and can be removed by alcoholic picric acid or alkaline alcohol.
10% aqueous formalin - ANSWER Very hypotonic and may produce formalin pigment.
10% formalin saline - ANSWER Isotonic yet still may produce formalin pigment.
Calcium Formalin (Calcium Chloride) - ANSWER Solution that preserves phospholipids in tissues.
Formalin Ammonium Bromide - ANSWER Formalin solution that is recommended for the CNS, Cajal astrocyte procedure. Lyses RBC, causes nuclei to give a direct positive Schiff reaction.
Acetate Formalin - ANSWER Alternative to buffered formalin solutions, yielding a 7.3 pH.
10% Neutralized Formalin - ANSWER Most widely used. May become acidic after withdraw from storage container and exposed to air.
10% Neutral Buffered Formalin - ANSWER Formalin solution uses sodium phosphate as a buffer and is recommended for routine use. Isotonic with a pH of 6.8.
Modified Millonig Formalin - ANSWER Formalin solution allows electron microscopy on stored tissue, less extraction of cellular elements.
Alcoholic Formalin - ANSWER In addition to fixation, this solution also begins the dehydration process. [Show Less]