Define what microorganisms are. ✔✔ living organisms that are too small to be observed using the naked eye
Compare and contrast the three domains of
... [Show More] life ✔✔ 1. Eukarya- Unicellular and Multicellular-May consist of one or more cells. Eukaryotic-Cells which contain a nucleus and internal complexity.
2. Bacteria- Unicellular-Consists of only one cell. Prokaryotic-Cells which have no nucleus and lack internal complexity.
3. Archaea- Unicellular-Consists of only one cell. Prokaryotic-Cells which contain no nulcleus and lack internal complexity. (Hint: this domain is closest to bacteria)
Define the terminology used in the study of fungi ✔✔
Describe the proper procedures for caring for, transporting, and storing a compound light microscope ✔✔ 1. Transport - grip the microscope arm with one hand and support the base with the other
2. Caring - clean each objective lens and ocular lens before and after using. Use kimwipe moistened with small amount of 70% ethanol.
Identify all parts of a compound light microscope and describe the function of each. ✔✔ 1. Resolving power - the ability of an optical device to produce separate images, focuses on the object.
2. Magnifying power - how much larger a lens can make the object appear, zooms in on the object.
Why is oil necessary when using the 100X objective ✔✔ Oil has the same refractive index as glass, the loss of light passing through the lens is minimized
Describe qualities of eukaryotic microorganisms. ✔✔ They have a nuclear membrane around their DNA. Their DNA is wound around histones. They have a complex membrane-bound organelles.
Compare and contrast characteristics of prokaryotes with those of eukaryotes. ✔✔
Define terminology used in the study of fungi ✔✔
List the properties of the growth medium used to grow the fungi (in exercise 2) ✔✔ Sabouraud's Dextrose Agar - this growth medium has a high sugar concentrations and a slightly acididc pH of 5.6 that make it unsuitable for the growth of bacteria.
Describe why lactophenol cotton blue stain is used when viewing a fungal specimen under a microscope ✔✔ The lactic acid and phenol components of this stain help to kill and preserve the organism, while cotton blue stains the chitin present in the cell wall
Why are fungi larger than bacteria? ✔✔ Fungi are eukaryotes which are 10 micrometers, or greater, because they have cell organelles, nucleus, and larger chromosomes in their cells. Where as bacteria, a prokaryote is only one or few micrometers because they lack what the eukaryotes have. [Show Less]