Clinical Chemistry I ASCP Review (467 Questions) With 100% Verified Answers
Which of the following lamps provides a continuous spectrum of radiant energy
... [Show More] in the visible, near IR, and near UV regions of the spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury vapor - A most common light source for photometry in the visible region. continuous spectrum (360-800 nm). near IR and UV.
Hydrogen and Deuterium (200-375 nm)
Mercury vapor- not a continuous spectrum, emitting radiation at specific wavelengths
Which of the following isolates light
within a narrow region of the spectrum?
A. Photomultiplier tube
B. Monochromator
C. Photovoltaic cell
D. Detector - B Use for selecting a narrow band wavelengths from a continuous spectrum. Three kinds (prisms, filters, diffraction gratings.
Which of the following is not descriptive of a photomultiplier tube?
A. Emits electrons proportionally to
initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - C
PMT- responds to the radiant energy light it absorbs by emitting electrons in a proportional amount to the initial light absorbed.
Which of the following is false about
a photomultiplier tube?
A. Converts radiant energy (light)
to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and
a layer of selenium - D is part of a barrier layer cell
PMT- 1. light to electricity
2. Amplification
5. Which type of photodetector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - D
256-2048 arranged in a linear fashion
Respond to a specific wavelength that results in a continuous UV/ visible spectrum.
When performing spectrophotometer
quality assurance checks, what is the
holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy - D
In spectrophotometric analysis, what is the purpose of the reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents - D
In regard to bichromatic analysis, which of the following is false?
A. Absorbance is measured at the
spectral absorbance peak for a blank
and the sample using the same
wavelength.
B. Eliminates background interferences
C. Sample concentration determined
from difference in two measured
absorbances
D. Functions as a reference blank for
each sample - A
The bandpass of a spectrophotometer is
10 nm. If an instalment is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what
wavelength range? [Show Less]