E. coli is commonly used for the bio-manufacturing of what? - CORRECT ANSWER insulin
and recombinant proteins
Advantages of using mammalian cells for
... [Show More] protein expression: - CORRECT ANSWER posttranslational modification, folding
What lines are frequently used for transfection for monoclonal antibodies/ therapeutic protein
expression? - CORRECT ANSWER Stable CHO lines
What is a hybridoma? - CORRECT ANSWER a hybrid mouse b-cell with a cancer cell line used
as the basis for the production of antibodies in large numbers
What is considered upstream processes? - CORRECT ANSWER Design of experiments, parallel
bioreactors, and fermentation
response surface methodology - CORRECT ANSWER a technique used to estimate the effects
of a combination of many independent variables without having to conduct a complete factorial
experiment combining all levels of all variables
Design of experiments: - CORRECT ANSWER NOT one factor at a time, uses RSM
What are considered downstream processes? - CORRECT ANSWER Centrifugation,
homogenization/filtration, chromatography
Continuous centrifugation - CORRECT ANSWER High dry solids, 4kg/cycle recovery
Microfiltration - CORRECT ANSWER cells/particles 1 e6 Daltons or more
Ultrafiltration - CORRECT ANSWER proteins 1 e6 Daltons or less
Diafiltration - CORRECT ANSWER ultrafiltration where a solution is added at the same time to
exchange buffer
Normal flow - CORRECT ANSWER traditional process using membrane filter disks (clogging
is common)
Depth filtrarion - CORRECT ANSWER utilizes a 3D filter for improved filtration and ease of
use (scaleable)
Tangental flow filtration - CORRECT ANSWER stackable membranes utilizing a membrane
lumen and pressure to allow filtration and reduce clogging
Chemical homogenization - CORRECT ANSWER detergents break membrane
Mechanical homogenization - CORRECT ANSWER high pressure and/or ballistics used to
break cells
ion exchange chromatography - CORRECT ANSWER molecules separated based on net surface
charge
Biotechnology job outlook - CORRECT ANSWER average wage is double public sector wages
PETase - CORRECT ANSWER a PET degrading enzyme found in some bacteria that breaks
PET down into its precursor molecules (ethylene glycol and terephthalic acid)
Goal of PETase project: - CORRECT ANSWER to express and purify the PETase, identify new
enzymes, and identify valuable compounds from byproducts
PETase resembles what enzyme - CORRECT ANSWER Cutinase
Bioprospecting - CORRECT ANSWER searching for organisms that might provide new
enzymes that degrade plastics
Bioprospecting steops - CORRECT ANSWER Screen samples, isolate pure cultures, sequence
isolates for taxonomy
EG Red - CORRECT ANSWER M. goesingense
Traditional remanufacturing of PET (Biorefinery) - CORRECT ANSWER enzyme produced
separately and added to substrate
Consolidated bioprocessing of PET - CORRECT ANSWER enzyme produced by degrading
organism which also generates final product
Steps of consolidated bioprocessing - CORRECT ANSWER enzyme production, substrate
hydrolysis, and fermentation
Q-PCR - CORRECT ANSWER quantitative PCR uses the strength of flourescence to determine
the abundance of a gene in a sample
CRISPR to detect COVID-19 - CORRECT ANSWER nuclease recognizes CoV2 RNA and
cleavage produces a detectable product [Show Less]