BOC Microtomy Exam - Questions and Answers Which of the following could be substituted for gelatin as an additive to the flotation bath? A.
... [Show More] Aminoalkylsilane B. Chromium potassium sulfate C. Poly-L-lysine D. Agar During microtomy, it is noted that a faced block has a central area that is soft and mushy. This tissue should have been: A. Chilled longer before facing B. Cut thicker at the grossing table C. Left in paraffin longer D. Reprocessed before embedding Lymph node tissue shatters when sectioned in a cryostat maintained at -20 C. The most appropriate action is to: A. Switch to an unused part of the blade B. Increase the blade tilt C. Chill the block with a spray coolant D. Warm the block slightly For good demonstration of myelin sheaths, paraffin sections should be: A. Floated on a cool water bath B. Cut 10 to 15 um thick C. Coated with celloidin D. Dried at room temp In paraffin microtomy, knowledge of the tissue type and possible disease process present in the tissue may be important because it may change the requirements in: A. Clearance angle B. Section thickness C. Flotation medium D. Blade temp Prominent peripheral chatter is obtained on sections of a paraffin block that has been routinely fixed and processed. This can probably be corrected by re-cutting the block: A. After soaking it in ice water B. Using a faster cutting speed C. After re-embedding in a different paraffin D. Using a greater clearance angle Slides used for mounting cryostat frozen sections should be kept at the same temp as the: A. Tissue B. Blade C. Operator D. Room Paraffin sections should be cut at 2 micrometers when studying: A. Basement membranes B. Myelin C. Amyloid D. Nerve fibers The tissue block fails to advance during the preparation of frozen sections. This probably could be corrected by: A. Changing the blade angle B. Replacing the blade C. Decreasing the chamber temp D. Cleaning and oiling the microtome An H&E stained lymph node section reveals overlapping nuclei. This indicates that the section is most likely: A. Over-stained with hematoxylin B. Too thick C. Of an abnormal node D. Appropriately sectioned The material of choice for immunofluorescence microscopy and enzyme histochemical studies is: A. Paraffin sections B. Cryostat sections C. Air-dried imprints D. Alcohol-fixed imprints When a femoral head is sectioned, the marrow portion sections satisfactorily but the cortex fragments. The most probable cause of this problem is that the: A. Decalcifying agent was too strong B. Embedding medium is too hard C. Decalcifying agent was not washed out D. Compact bone is underdecalcified A fingernail has been fixed in formalin, routinely processed, and embedded in paraffin. The tissue is very hard and sections are difficult to obtain. Sectioning quality will be improved and tissue components will be BEST preserved and demonstrated by gently facing the block and: A. Soaking in water B. Warming the block slightly C. Soaking in a solution that softens keratin D. Treating with decalcifying fluid Nuclear bubbling artifact may be cause by: A. Leaving sections too long on the flotation bath B. Using too much adhesive in the flotation bath C. Placing the slide in a hot dryer without allowing to drain D. Sectioning too rapidly According to the Histology Quality Improvement Program, the most common microtomy artifact is: A. Wavy sections B. Holes in the section C. Air bubbles D. Wrinkles and folds Of the following, the most common cause of tears and/or section fragmentation is: A. Incomplete fixation and processing B. Flotation bath too cold C. Dull microtome blade D. Worn microtome parts The use of coolant sprays during sectioning may result in: A. Wavy sections B. Fragmented sections C. Parched earth appearance D. Chatter or micro-vibration A very serious microtomy artifact in the histopathology lab is: [Show Less]