What would be the best positive control for the transformation?
different plasmid with the same backbone
What happens if we grow the bacteria in a
... [Show More] medium containing chloramphenicol?
cells with the plasmid will survive
What is the purpose of the antibiotics in the agar plate?
selection
What is the green gene on our plasmid (amilCP)?
reporter gene
What would the sticker ends of your BioBrick part look like if you cut our plasmid with Xbal (T'CTAGA) and Psti (CTGCA'G)?
CTAG and TGCA
Which restriction enzyme produces the same overhand as Xbal?
Spel
Which of the following sticker ends would be fused to your BioBrick part by the ligase?
5' CTAGTAGC (BioBrick part 2)
3' ATCG (BioBrick part 2)
(BioBrick part 1) GCTT 3'
(BioBrick part 1) CGAAGATC 5'
Which of the following restriction enzymes could be ligated together?
Xbal and Spel
Why do we heat the tube to 80 C?
inactivate enzyme
Now our parts are cut. How can we combine them together?
use a ligase
What is NOT an advantage of the 3A assembly?
easy gel purification
What is your observation when looking at the two images?
increased number of stained blood vessels (brown) in the ductal carcinoma
What does the signaling protein VEGF stimulate?
mitosis
Why is cancer growth dependent on an increased number of blood vessels?
to supply the tumor with oxygen and nutrients
How can we easily determine VEGF expression in a western blot experiment?
by using a primary antibody targeting VEGF
In addition to the anti0VEGF antibody, Marie has decided to also add a VEGFR primary antibody. At which position do you expect the band for VEGFR to appear? Remember that in SDS-PAGE proteins are separated according to their molecular weight.
VEGFR runs at a higher molecular weight than WEGF
Looking at the western blot result. What is your conclusion for the patient samples?
VEGF and VEGFR are overexpressed
As you can see, Marie also added an anti-beta-actin antibody. Why do you think this was important?
to check if the same amount of sample was loaded on the gel
In which other type of cancers do you expect VEGF signaling to play a role?
in the progression of most solid tumors
What property prevents the ligands of cell surface receptors from entering the cell? Remember that the ligands are usually secreted into the extracellular fluid or the blood-stream.
hydrophobicity
What does a target cell require to respond to an extracellular signal molecule?
-access to the ligand
-matching receptors
-activation of effector proteins [Show Less]