Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4
... [Show More] Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
Lab 1 Notebook
Back to Home Page
Lab 1 Title: Keeping a lab notebook
Objective: To establish an organized template for keeping experimental records, procedures
and results.
Procedure:
1. This is where each step of the given protocol is recorded.
2. Be sure to clearly indicate any deviations that may occur during the experiment.
Notes: Additional (helpful) information placed here.
Results: A summary of the final outcome of the experiment should be described here.
Lab 2 Notebook
Back to Home Page
02 Title: Basics of microscopy
Objective: To become familiar with the basic component of a light as well as how to load a
sample for viewing.
Procedure:
1. Review parts and components
2. Load sample slide onto microscope
3. Select magnification
4. Make necessary adjustments to optimize sample visualization
Notes: basic parts of a microscope: eyepiece, arm/neck, objectives (different magnification
lenses 4x, 10x, 40x, 100x), revolving nosepiece, stage (where samples are placed, using a
clamp holder.), stage controls, coarse/fine focus, iris diaphragm, light source, base.
Using a standard light microscope which uses a halogen light bulb. Only grab the microscope
by the arm/neck to move it. A lot of eyepieces have a pointer.
objectives (different magnification lenses 4x, 10x, 40x, 100x) two types-dry vs oil, intensity of
light source: too bright-saturation, too dark-low visibility. [Power of objective] x [power of
eyepiece]= Total magnification
-if a cell is 15mm in diaqmeter, using a 40x objective and a 5x eyepiece the cell will now
appear to the eye 200 times larger (200x) or 3000mm in diameter.
Results: Magnification= objective x eyepiece
Lab 3 Notebook
Back to Home Page
03 Title: Mounting Techniques
Objective: Microscopic examination of bacterial samples through various staining techniques.
Identify color and shape of given samples.
Procedure:
Dry Mount
1. Clean glass slide (70% ethanol)
2. Circle area on slide for easy location of specimen(optional-with a sharpie on the b
bottom side of slide so you don’t contaminate sample)
3. Apply organism to slide
-if from culture, use sterile loop to spread onto slide
-If from plate, use sterile loop to pick colonies and mix with a drop of distilled water
4. Air dry at room temperature until all moisture has evaporated
Wet Mount
1. Clean glass slide (70% ethanol)
2. Circle area on slide with a wax/hydrophobic pen(keeps water inside the ring of wax)
3. Apply organism to the slide
-if from culture, use sterile loop to spread onto slide
-If from plate, use sterile loop to pick colonies and mix with a drop of distilled water [Show Less]