Which of the following is not found in DNA?
A. Oxygen
B. Phosphate
C. Nitrogen
D. Sulfur
D. Sulfur
In the 'Hershey Chase experiement' which
... [Show More] macromolecule did the bacteriophage inject into the bacterium?
A. DNA
B. RNA
C. protein
D. Fatty acids
A. DNA
True or false: The Hershey and chase experiment demonstrated that DNA was a double helix
False
What functional group differentiates RNA from DNA?
A. Carbonyl
B. Hydroxyl
C. Methyl
D. Sulfhydryl
B. Hydroxyl
Imagine the following sequence was one strand in a double helix: GGATCGAGCGATA. How many hydrogen bonds would form between this sequence and the sequence on the opposite strand?
33
Hershey-Chase Experiment
Used radioactive material to label DNA and protein; infected bacteria passed on DNA; helped prove that DNA is genetic material not proteins
How many hydrogen bonds are between A and T?
2 hydrogen bonds
How many hydrogen bonds are between G and C?
3 hydrogen bonds
Explain the following statement: DNA is synthesized in a 5' to 3' direction. One sentence please.
DNA polymerase adds new nucleotides to the 3'OH group of the deoxyribose sugar.
If you have 23% T nucleotides in an entire genome, how many C nucleotides should be present?
27
Describe 3 pieces of evidence that helped demonstrate DNA's properties. One sentence each.
1. Chargaff showed the %A = %T and %C = %G.
2. Rosalind Franklin used X-ray crystallography to show the 3-D structure of DNA.
3. Watson and Crick used mathematical modeling to deduce the completed 3D double helix.
4. Hershey and Chase showed that DNA was the heritable material. 5. Messelson and Stahl showed that DNA replication was semi-conservative.
Describe Rosalind Franklin's impact on early DNA research. HINT: there is link to a video about Franklin on Monday's slides. Two sentences please.
She used X-ray crystallography to document the structure of DNA. However, her findings were improperly shared with Watson and Crick who then used them to develop their model of DNA structure.
TRUE OR FALSE: Did Watson and Crick discover DNA?
False
Which atom did Messelson and Stahl use to track daughter strands of DNA?
a. phosphorous
b. nitrogen
c. sulfur
d. oxygen
b. nitrogen
Messelson-Stahl Experiment
Brainstormed 3 possible models (conservative, semiconservative, and dispersive) to explain DNA replication. Conducted an experiment to determine which one was correct. They used isotopes of nitrogen to tell old DNA and new DNA apart. new DNA was lighter bc it was prepared with lighter nitrogen and the old was prepared with heavy nitrogen. (Processes explained in specific flashcards)
What type of replication did Messelson and Stahl demonstrate that cells use?
a. conservative
b. dispersive
c. semi-conservative
d. none of the above
c. semi-conservative
How many replication forks are there at a replication bubble?
2
Describe what is meant when we say 'DNA is anti-parallel'? One sentence please.
If one strand has a 5' on the left and a 3' on the right - the strand it is hydrogen bonded to will have a 5' on the right and a 3' on the left.
Which of the follow proteins does not play a role in the initial unwinding and stabilization of DNA during replication?
a. DNA polymerase
b. Helicase
c. Single-stranded DNA binding proteins
d. Topoisomerase
a. DNA polymerase
Which of the following enzymes relieves tension during DNA replication?
a. Helicase
b. Topoisomerase
c. DNA polymerase
d. Ligase
b. topoisomerase [Show Less]