Alberts Molecular Biology of Cell - Chapter 008 Test Quiz Bank With Questions & Answers 2023/2024 Verified
hybridoma correct answers Cell line used in
... [Show More] the production of monoclonal antibodies. Obtained by fusing antibody-secreting B cells with cells of a B lymphocyte tumor
monoclonal antibody correct answers Antibody secreted by a hybridoma cell line. Because the hybridoma is generated by the fusion of a single B cell with a single tumor cell, each hybridoma produces antibodies that are all identical
affinity chromatography correct answers Type of chromatography in which the protein mixture to be purified is passed over a matrix to which specific ligands for the required protein are attached, so that the protein is retained on the matrix
fusion protein correct answers Engineered protein that combines two or more normally separate polypeptides. Produced from a recombinant gene
high-performance liquid chromatography correct answers Type of chromatography that uses columns packed with tiny beads of matrix; the solution to be separated is pushed through under high pressure
fluorescence resonance energy transfer correct answers Technique for monitoring the closeness of two fluorescently labeled molecules (and thus their interaction) in cells
nuclear magnetic resonance spectroscopy correct answers _____ is the resonant absorption of electromagnetic radiation at a specific frequency by atomic nuclei in a magnetic field, due to flipping of the orientation of their magnetic dipole moments. The _______ spectrum provides information about the chemical environment of the nuclei. _______ is used widely to determine the three-dimensional structure of small proteins and other small molecules. The principles of ______ are also used for medical diagnostic purposes in magnetic resonance imaging
SDS polyacrylamide-gel electroophoresis (SDS-PAGE) correct answers Type of electrophoresis used to separate proteins by size. The protein mixture to be separated is first treated with a powerful negatively charged detergent and with a reducing agent (beta mercaptoethanol), before being run through a polyacrylamide gel. The detergent and reducing agent unfold the proteins, free them from association with other molecules, and separated the polypeptide subunits
surface plasmon resonance correct answers Technique used to characterize molecular interactions, such as antibody-antigen binding, ligand-receptor coupling, and the binding of proteins to DNA. Binding interactions are detected by monitoring the reflection of a beam of light off the interface between an aqueous solution of potential binding molecules and a biosensor surface carrying the immobilized bait protein
two-hybrid system correct answers Molecular genetic technique for identifying protein-protein and protein-DNA interactions
Western blotting (immunoblotting) correct answers Technique by which proteins are separated by electrophoresis and immobilized on a paper sheet and then analyzed, usually by means of a labeled antibody
x-ray crystallography correct answers Technique for determining the three-dimensional arrangement of atoms in a molecule based on the diffraction pattern of X-rays passing through a crystal of the molecule
cDNA clone correct answers DNA molecule made as a copy of mRNA and therefore lacking the introns that are present in genomic DNA
cDNA library correct answers Collection of cloned DNA molecules representing complementary DNA copies of the mRNA produced by a cell
dideoxy method correct answers The standard enzymatic method of DNA sequencing
expression vector correct answers A virus or plasmidthat carries a DNA sequence into a suitable host cell and there directs the synthesis of the protein encoded by the sequence
genetic engineering correct answers Collection of techniques by which DNA segments from different sources are combined to make a new DNA, often called a recombinant DNA. Recombinant DNAs are widely used in the cloning of genes, in the genetic modification of organisms, and in molecular biology generally
genomic DNA clones correct answers represent DNA cloned directly from chromosomal DNA
genomic DNA library correct answers a collection of DNA cloned directly from chromosomal DNA from a given genome [Show Less]