. What is the complementary sequence for the following DNA sequence? 5'
- GCC ATC TCG AAT - 3': 5' - ATT CGA GAT GGC - 3'
2. Assuming 100% reaction
... [Show More] efficiency, how many DNA copies are created
after the completion of four complete PCR cycles?: 16
3. What is the function of DNA polymerase in the process of PCR?: It recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.
4. What are possible blood types of the parents of a patient with blood type
AB?: A and B
5. Studies have shown that increased expression of two genes called SIRT1
and SIRT2 is associated with chronic exposure to cocaine and contributes to
drug addiction. What difference in the regions of the SIRT1 and SIRT2 genes
in people addicted to cocaine increases their expression?: The nucleosomes
become more widely spaced.
6. Mice with low levels of agouti gene expression tend to have yellow fur
and are obese, while mice with higher levels of expression are brown and of
normal weight. Studies show that expression of the agouti gene in mice can
be controlled by epigenetics. What scenario describes epigenetic regulation
of the agouti gene?: Mother mice fed methyl-rich diets during pregnancy give
birth to offspring with decreased agouti gene expression compared to those fed a
methyl-poor diet.
7. Oxidative damage to DNA can often result in the alteration of a single
nucleotide. Which DNA repair mechanism would repair this type of damage?-
: The mutation rate of all other genes is higher due to failure to repair.
8. Which reaction is responsible for the formation of polypeptides?: Dehydration
9. Which term describes the order of amino acids in a peptide chain?: Primary
structure
10. When using heat to denature a protein, which force is the first to be
disrupted as temperature increases?: Hydrophobic interactions
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11. A commonly used technique for protein denaturation is adding an acid
or a base to dramatically change the pH. Which force is most impacted by
this change in pH?: Ionic bonds [Show Less]