WGU Biochemistry OA Study Notes
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• Hemophilia pedigree- father has hemophilia, mother does not. Outcome for kids?
His daughters would be carriers, this is x-linked recessive.
• X-linked dominant: an affected male will have 100% of his daughters affected and 0% of his sons.
• Pedigree chart: only one is autosomal dominant
Autosomal= males and females are EQUALLY affected. Dominant= non carrier parents.
• Punnett square question: 4 squares w. drop down boxes, select the correct answer for each one. They put the dead puppy in there to throw you off!!!!!! Look instead at the three remaining puppies
Complete dominance: either 3 black and 1 white
Co-dominance: black, white, and 2 SPOTTED WITH BOTH
Incomplete dominance: black, white and 2 GREY
• PCR *** Know the steps of PCR*** one question asks about the first step
Polymerase Chain Reaction= the process of copying DNA in the lab.
With PCR you need:
1. Template DNA
2. Nucleotides (dNTPS)
3. DNA polymerase
4. DNA primers
Steps of PCR
1. Denaturation (heated to 95C to separate it)
2. Annealing (reaction is cooled to 50C, primers stick to the DNA that you want to copy and ADD DNA polymerase
3. ELONGATION (reaction heated to 70C and DNA polymerase add nucleotides to building new DNA Strand
“Have An Éclair” à Heat Annealing Elongation
▪ For each round of PCR there are 2 created, continue to multiply by 2
1x2=2 2x2=4 4x2=8 8x2= 16
o Ligase is NOT involved in PCR
o Remember the first step is denaturation / heating
• Base Excision Repair, know about it
This is how you repair a mutation. Base excision repair is used to repair damage to bases caused by harmful molecules. You removed the base that is damaged and replace it. DNA Glycosylase see’s the damaged DNA and removes it. Then DNA polymerase puts the right base back in while DNA ligase seals it back up. Boom all fixed.
• Base excision repair (BER) removes a single nucleotide!!!!!! Only one base camp!
• Mismatch repair is the only one to occur during REPLICATION— DURING THE PROOFREADING.
o During replication, DNA polymerase proofreads, but sometimes
a mismatch occurs. So MMR removes a LARGE section of the nucleotides from the new DNA, DNA polymerase tries again.
o Know what damage MMR repairs in DNA: G-C A-T
• NucleoTIDE excision repair- TIDE like the beach, sun exposure--- UV damage repair [Show Less]