Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids?
Correct Answer-His daughters would be carriers. This
... [Show More] is x-link recessive.
Autosomal:
Dominant: Correct Answer-Autosomal: males and females equally affected.
Dominant: non-carrier parents
polymerase chain reaction (PCR) Correct Answer-The process of copying DNA in the lab. Uses Template
DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers.
3 Steps of PCR Correct Answer-1. Denaturation: DNA is heated to 95C to separate it.
2. Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy and add DNA
polymerase.
3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides building a new DNA
strand.
Base Excision Repair (BER) Correct Answer-How you repair a mutation. BER is used to repair damage to a
base caused by harmful molecules. You remove the base that is damaged and replace it. *BER removes a
single nucleotide*
DNA glycolsylase - sees damaged DNA and removes it.
DNA polymerase-puts the right one back in while DNA ligase seals it.
Mismatch repair (MMR) occurs during: Correct Answer-replication. DNA polymerase proofreads but
sometimes a mismatch pair gets through. MMR removes a large section of the nucleotides from the new
DNA and DNA polymerase tries again. (Ex: C-T instead of C-A)
Mismatch Repair corrects what kind of DNA damage? Correct Answer-When a base is mismatched due
to errors in replication. Such as G-T instead of G-C. DNA polymerase comes by and fixes it.
What happens when DNA polymerase binds to DNA to make RNA? Correct Answer-TRANSCRIPTION!
DNA polymerase takes the individual nucleotides and matches them to the parental sequences to
ensure a correct pair. It must bind with RNA primer to work.
What is needed for DNA replication? Correct Answer-DNA polymerase
Nonsense Mutation Correct Answer-Change in 1 nucleotide produces a STOP codon Stop= nonsense
because it is no more.
Silent Mutation Correct Answer-Change in 1 nucleotide but codes for the same amino acid. Silent= the
change doesn't change the name of the protein
Missense Mutation Correct Answer-Change in 1 nucleotide leads to a code for a different amino acid.
Missense = mistake was made.
What happends during RNA splicing? Correct Answer-During RNA splicing introns are cut out, the
remaining exons are joined together.
5'ATG AGT CTC TCT 3'
Find the DNA template strand. Correct Answer-3'TAC TCA GAG AGA 5' [Show Less]