Triple Sugar-Iron Agar Test Questions with Verified Answers
Gram- - CORRECT ANSWER Is TSI gram+ or gram-
used to differentiate members of
... [Show More] Enterobacteriacea and to distinguish them from other gram- rods - CORRECT ANSWER What is the application of TSI?
1 glucose, lactose & sucrose 2 animal protein 3 ferrous sulfate 4 sodium thiosulfate 5 phenol red - CORRECT ANSWER What is the media in TSI (triple sugar iron)
carbohydrate for fermentation - CORRECT ANSWER What is the purpose of glucose, lactose & sucrose
source of carbon & nitrogen - CORRECT ANSWER What is the purpose of animal proteins?
source of oxidized sulfur & hydrogen sulfide indicator - CORRECT ANSWER What is the purpose of Ferrous sulfate?
source of oxidized sulfur - CORRECT ANSWER What is the purpose of sodium thiosulfate?
pH indicator - CORRECT ANSWER What is the purpose of phenol red?
glucose & lactose and/or sucrose fermentation with acid accumulation in slant and butt - CORRECT ANSWER Yellow slant/ yellow butt
glucose fermentation with acid production. Proteins catabolized aerobically (in the slant) with alkaline products (reversion) - CORRECT ANSWER Red slant/ yellow butt
no fermentation. peptone catabolized aerobically and anaerobically with alkaline products. not from enterobacteriaceae - CORRECT ANSWER red slant/ red butt
no fermentation. peptone catabolized aerobically with alkaline products. not from enterobacteriaceae - CORRECT ANSWER red slant/ no chang ***********
organism is growing slowly or not at all. Not from enterobacteriaceae - CORRECT ANSWER no change in slant/ no change in butt
sulfur reduction - CORRECT ANSWER black precipitate in the agar
gas production - CORRECT ANSWER cracks in or lifting of agar
the color of the slant determines which carbohydrates have been fermented - CORRECT ANSWER It the butt of an inoculated tube is totally black, what color do you assume the butt is?
thiosulfate, the breakdown of cysteine in the peptone - CORRECT ANSWER Hydrogen sulfide may be produced by the reduction of ______ in the medium or by _______________.
The microorgansim utilizes peptone aerobically & anaerobically and amino acids will alkalinize the medium and turn it red in the slant and butt. - CORRECT ANSWER It the medium has turned red, (slant and butt) what has happened?
Enterobacteriaceae - CORRECT ANSWER TSI is used to primarily differentiate member of what family?
enterobacteriaceae, bacilli - CORRECT ANSWER The triple sugar-iron agar test is used to distinguish between members of the genera _________, and between them and other groups of intestinal _______.
carbohydrate fermentation, hydrogen sulfide - CORRECT ANSWER It is a screening procedure for ___________ and _____________ production
1, lactose, sucrose, 0.1, glucose - CORRECT ANSWER TSI agar slants contain __% _______ and _______ and ___% _________.
phenol red - CORRECT ANSWER Acid production during carbohydrate fermentation can also be detected by the presence of the acid-base indicator _________.
stab-and-streak, 18, 24 - CORRECT ANSWER determined by using a __________ procedure and is interpreted between ___ and ___ hours
alkaline, red, acid, yellow, - CORRECT ANSWER ______ slant (_____) and ______ butt (____) with or without gas production (breaks ***********) = only glucose fermentation has occurred.
glucose, acid - CORRECT ANSWER these organisms preferentially degrade ________ first, which is present in very low concentration and produces ______
oxidation, glucose, ammonia, peptones - CORRECT ANSWER The alkaline slant is the result of the rapid ________ of of the small amount of acid produced on the surface of the slant by the fermentation of _______ and the production of __________ by the degradation of ________.
oxygen tension, growth - CORRECT ANSWER the acid butt is due to reduced oxidation of acids because of the lower __________ as well as slower _______ of the organism
acid, yellow, yellow - CORRECT ANSWER ______ slant (________) and butt (_________) with or without gas production = lactose and/or sucrose have been fermented. The higher concentration of both of these sugars results in the continuous fermentation on the slant and ***********
alkaline, red, peptones, ammonia, aerobic - CORRECT ANSWER _________ slant and butt (_____) = carbohydrate fermentation has not occurred. ________ are catabolized in place of carbohydrates and ________ is produced. It can also be determined whether the fermentation was aerobic or anaerobic. if only ________ catabolism occurred then only the slant will be alkaline. if aerobic and anaerobic has occurred then the slant and butt will be alkaline
sodium thiosulfate, iron sulfate - CORRECT ANSWER the capability of certain microbes to produce gaseous hydrogen sulfide can be detected by the presence of __________. __________ is added to the medium to allow detection of the colorless gas
black - CORRECT ANSWER the combination of hydrogen sulfide with ferrous sulfate produced iron sulfide, an insoluble _____ precipitate that colors the whole butt
Triple Sugar Iron Test - CORRECT ANSWER What is the name of this test?
Differentiate between gram-negative rods - CORRECT ANSWER What is the purpose of this test?
Slant - Acid or Alkaline
Butt - Acid or Alkaline
Gas Production - + or -
Hydrogen Sulfide Production - + or - - CORRECT ANSWER What is the order of the results of this test?
Phenol Red - CORRECT ANSWER What is the pH indicator for carbohydrate fermentation?
Ferrous Sulfate - CORRECT ANSWER What is the color indicator for hydrogen sulfide production?
Sodium Thiosulfate - CORRECT ANSWER What is the substrate for hydrogen sulfide production?
Glucose
Lactose
Sucrose
Peptones - CORRECT ANSWER What are the substrates for carbohydrate fermentation?
Yellow
Tube 1 - CORRECT ANSWER What are the colors for an acidic reaction on the slant?
Which tubes have an acidic reaction on the slant?
Pink or Orange
Tubes 2, 3 and 4 - CORRECT ANSWER What are the colors for an alkaline reaction on the slant?
Which tubes have an alkaline slant?
Black or Yellow
Tubes 1, 2 and 4 - CORRECT ANSWER What are the colors for an acidic reaction on the butt?
Which tubes have an acidic reaction on the butt?
Pink or Orange
Tube 3 - CORRECT ANSWER What are the colors for an alkaline reaction on the butt?
Which tubes have an alkaline butt?
(Slant) Acid / (Butt) Acid / (Gas) + / (H2S) + - CORRECT ANSWER In a TSI slant, if the slant has turned yellow and the butt has turned black with bubbles in the media, what is recorded?
(Slant) Alk / (Butt) Acid / (Gas) - / (H2S) + - CORRECT ANSWER In a TSI slant, if the slant has turned pink and the butt has turned black, what is recorded?
(Slant) Acid / (Butt) Acid / (Gas) + / (H2S) - - CORRECT ANSWER In a TSI slant, if the slant has turned yellow and the butt has turned yellow with bubbles in the media, what is recorded?
(Slant) Alk / (Butt) Alk / (Gas) - / (H2S) - - CORRECT ANSWER In a TSI slant, if the slant has turned pink and the butt has turned pink, what is recorded? [Show Less]