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Exam (elaborations) TEST BANK FOR TEXTBOOK OF DIAGNOSTIC MICROBIOLOGY 4TH EDITION
Chapter 04: Control of Microorganisms
Test Bank
MULTIPLE CHOICE
1. The chemical or physical method that destroys all forms of life is called:
a. Sterilization
Textbook of Diagnostic
Microbiology 4th Edition Test
Bank
b. Disinfection
c. Bacteriostatic
d. Bactericidal
ANS: A
Sterilization is the destruction of all forms of life, including bacterial spores. Disinfection is a process
that eliminates a defined scope of microorganisms, including, in some cases, spores. Bacteriostatic
inhibits the growth of microorganisms. Bactericidal kills bacteria.
REF: page 62 OBJ: Level 1 – Recall
2. Organisms that are the most resistant to heat, chemicals, and radiation are:
a. Parasites
b. Prions
c. Bacteria
d. Viruses
ANS: B
Prions are naked pieces of protein, so they are harder to kill than any other organism. Viruses
usually contain a nucleic acid, and all the mentioned forms of killing can effectively disrupt their
nucleic acid. Bacteria and parasites are complete organisms that are killed by disinfection and
sterilization, even in the spore and cyst stages.
REF: page 63 OBJ: Level 1 – Recall
3. After using the phone, the laboratory tech sprayed the receiver with a chemical spray. This
process will kill a defined scope of microorganisms. What is this process called?
a. Sterilization
b. Bacteriostatic
c. Disinfection
d. Bactericidal
ANS: C
Sterilization kills all organisms and spores at a site. Bacteriostatic and bactericidal are adjectives that
describe the particular action of chemical agents: to inhibit bacterial growth or kill bacteria.
Disinfection kills a defined scope of microorganisms.
REF: page 62 OBJ: Level 2 – Interpretation
4. Before performing a phlebotomy, the phlebotomist will clean the area on a patient’s arm with
a substance before inserting the needle. This substance is called a(n):
a. Disinfectant
b. Sterilizer
c. Antiseptic
d. Bactericidal
ANS: C
A disinfectant is a chemical agent used to kill microorganisms on an inanimate object. To sterilize is
to kill all life; skin will still have organisms growing after wiping. Bactericidal is the process of killing
bacteria. An antiseptic is a substance applied to the skin for the purpose of eliminating or reducing
the number of bacteria present.
REF: page 62 OBJ: Level 2 – Interpretation
5. All the following factors play a significant role in the selection and implementation of the
appropriate method of disinfection EXCEPT:
a. Temperature
b. Contact time
c. Biofilms
d. Humidity
ANS: D
Temperature, contact time, and biofilms all play a role in selection and implementation of the
appropriate method of disinfection. Humidity is not important when attempting to disinfect or kill
organisms.
REF: pages 62-63 OBJ: Level 1 – Recall
6. When eliminating organisms from inanimate objects, higher numbers of organisms require
longer exposure times because:
a. All disinfecting agents are not alike and some require shorter times.
b. The chemical composition of the disinfecting agent varies.
c. Disinfecting agents containing carbon tetrachloride require longer times to act.
d. It takes longer to eliminate 99% of microorganisms.
ANS: D
When there are higher numbers of microorganisms, it takes longer to kill 99% of microorganisms
present. Although disinfectants are different, it still takes longer to kill more organisms. The chemical
composition of a disinfecting agent may affect the time required to kill microorganisms, but microbial
load is a determining factor.
REF: page 63 OBJ: Level 1 – Recall
7. When using disinfectants, it is important to follow the manufacturer’s instructions for diluting
the product because if it is not diluted properly:
a. There may not be enough free chemicals to kill the organisms.
b. You would be wasting money by using too much disinfectant.
c. The resulting solution would not kill mycobacteria.
d. The resulting solution would not kill parasites.
ANS: A
The manufacturer tests disinfectants at a particular dilution, and this is the dilution needed for
optimum performance of the disinfectant. If the manufacturer’s instructions are not followed,
optimum effectiveness cannot be guaranteed.
REF: page 63 OBJ: Level 1 – Recall
8. If this is present on a surface to be disinfected, it can shield microorganisms from the
disinfectant or inactivate the disinfectant. What is this substance?
a. Bleach (sodium hypochlorite)
b. Organic material
c. Hydrochloric acid
d. Water
ANS: B
Bleach, hydrochloric acid, and water can counteract another disinfectant, but they cannot shield
microorganisms from a disinfectant. Organic matter (e.g., blood, pus) can keep the disinfectant from
reaching and killing the microorganism.
REF: page 64 OBJ: Level 1 – Recall
9. When killing microorganisms, you must take all the following into consideration EXCEPT:
a. A type of microorganism
b. Temperature
c. Humidity
d. Bioburden
ANS: C
The type of microorganism (bacteria, mycobacteria, parasite, etc.), the temperature, and the
bioburden (the number of bacteria) must be taken into consideration when decided how to best kill
microorganisms. The humidity plays no role in the killing of microorganisms.
REF: page 64 OBJ: Level 1 – Recall
10. Disinfectants are usually used at this temperature.
a. 50° to 100° C
b. 0° to 10° C
c. 25° to 50° C
d. 20° to 22° C
ANS: [Show Less]