MCB 3020C Module 2 Exam Review Questions and Answers 2024.
*Anything denoted in red are extra important takeaways
1. What is the function of the Z
... [Show More] ring in binary fission?
A. It forms a contractile ring at the septum => Made up of FtsZ (tubulin
homologues) molecules important for bacterial septation
B. It controls the replication of DNA => Replisome contains DNA replication
machinery
C. It separates the newly synthesized DNA molecules => ParA
polymerizes/depolymerizes to attach to ParB which recognizes the DNA
Sequence parS which is next to the origin of replication
D. Both A and B
E. Both B and C
2. Which of the following was the first broad-spectrum antibiotic discovered?
A. Salvarsan => First Synthetic antibiotic
B. Chloramphenicol => Made by a bacterium
C. Penicillin => First True antibiotic
D. Oxazolidinone => Considered new “drug of last resort”
E. Vancomycin
MCB 3020C Module 2 Exam Review
Questions and Answers 2024.
3. Which of the following is true about biofilms? (select all that apply)
A. Only bacteria of similar strains can form biofilms => Composed of many
different microorganisms
B. Heterogeneity among the bacteria can lead to increased interactions and
communication => Microbes with varying diversity communicate heavily in
biofilms via quorum sensing
C. Microbes that grow attached to the surfaces of these biofilms are planktonic
=> They are sessile, think plankton are planktonic; they are freely floating
D. The most metabolically active cells are located at the base of the biofilm =>
Slow growers near the base of the biofilm (Ch. 7 slide 35) not as metabolically
active but still active
E. They often have an EPS which provides structural and functional support
=> Extracellular polymeric substance makes biofilms tough to remove Ex.
Biofilms on surface of teeth can cause damage in the form of cavities and tooth
decay; have to scrape it off in a dental procedure. Conventional cleaning agents
and antibiotics can’t get past the EPS barrier.
4. Which of the following is true in relation to the central dogma of biology?
A. DNA is the template for its own replication => Depends on the holoenzyme
DNA polymerase
B. The flow of genetic information goes from DNA to RNA to proteins
C. mRNA is the template for protein translation
D. Both A and C
E. All of the above => (Ch. 13 slide 2)
5. In regard to proteins: => Made up of single or multiple polypeptides, contain
covalent and non-covalent bonds
A. Denaturing results in the loss of secondary structures => Denaturing results in
a protein losing all its structures except their primary structure; Ex. increasing
temperature or altering pH
B. They grow from the N-terminus to the C-terminus => Starts from the North/ Nterminus (where cleavage also takes place with signal peptides in order to
activate proteins)
C. All proteins can have a quaternary structure
D. Both A and B
E. Both B and C
6. Which of the following is false regarding bacterial horizontal gene transfer?
A. Bacterial transformation requires bacteria to be competent => DNA taken up
from environment
B. Bacterial conjugation requires a form of cell-cell contact to take place, usually
through a sex pilus => Sex pilus for conjugation NOT T4P (type IV pilus); Sex
pilus can transfer R plasmid
C. It can involve the transfer of genetic material from parent to offspring => This
is vertical gene transfer
D. Bacterial transduction involves phages inserting into the host’s genome
E. All of the above are true
7. Which of the following is false regarding penicillin?
A. It can activate autolysins => Breaks bonds in peptidoglycan which allows for
easy passage for NAM and NAG + new addition of them and assists with cellular
growth (Needs to be heavily regulated). Penicillins can further activate these to
lyse the bacterial cell
B. Organisms that produce β-lactamase can become resistant to penicillin =>
Penicillinase
C. Penicillin V and G are narrow spectrum and only work on Gram-positives =>
Ampicillin is semi-synthetic and has a broader spectrum than V and G
D. It is responsible for blocking peptide elongation => Deals with protein
synthesis inhibition not involved with penicillin (cell wall inhibitor)
E. It is a true antibiotic that is made by a fungus => Penicillium (fungi). [Show Less]