(MB) ASCP Practice Exam 2023/2024 -150 Questions and Answers
Which of the following is not a component of a nucleotide?
Phosphate
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Anti-codon
Ribose sugar
Nitrogen base - correct answers Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - correct answers Thymine
What genes would be screened in a breast cancer panel? - correct answers HER2, ERBB2,
BRCA1
Next Generation Sequencing uses: - correct answers Short sequence reads
Purines and pyrimidines differ from each other in that: - correct answers Purines have two
rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend on
which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers Initiation, exponential,
plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a singlegene disorder may be the cause of those clinical manifestations. A blood specimen was then sent
to your clinical laboratory for mutation screening in the Huntington gene. Testing with standard
PCR indicates that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answers 85 CAG repeats in the Huntington
gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answers 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - correct answers Helicase
Mutation in what gene is associated with Fragile X syndrome? - correct answers FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - correct answers t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": - correct
answers Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA
on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - correct answers Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK - correct answers BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - correct answers 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - correct answers Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a singlegene disorder may be the cause of those clinical manifestations. A blood specimen was then sent
to your clinical laboratory for mutation screening in the Huntingtin gene. Which of these
methods would best accomplish this task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - correct answers Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period greater
than seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - correct answers -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific exon, say
exon 11, of that gene then you cut it with restriction enzyme W. In a person without the mutation,
cutting the gene with restriction enzyme W generates two fragments of sizes, 100 bp and 250 bp.
Suppose a C>T mutation in gene X deletes a restriction site, yielding a fragment of 350 bp. You
would expect a heterozygous person for gene X to have these fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp - correct answers m/+ = 350 bp; 250 bp; 100 bp
Which of the following will more likely lower stringency conditions in the washing step of a
hybridization experiment?
Increase the concentration of salt in the wash solution buffer
Increase the temperature from, say 68°C to 75°C
Use a probe with a higher density of GC base pairs as compared to one with a lower GC base
pair density
Remove formamide from the wash solution buffer - correct answers Increase the
concentration of salt in the wash solution buffer
What enzyme is involved in LCR? - correct answers DNA Ligase
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