Access Code (located on the lid of your lab kit): AC- S40MUQO
Lab Report Format Expectations
Utilize college level grammar and professional formatting
... [Show More] when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. What is the purpose of Lab 8? What concepts is it trying to convey to a microbiology student?
This experimentation aims to serve the accumulated understanding and mastery gained
throughout the course to determine an unidentified microorganism via various difficulties. The
immediate purpose is to support comprehension of microbial identification methods, which
utilize a dichotomous key. The lab is designed to improve learners' mastery in systematically
selecting the species of an unidentified microorganism.
2. What is the purpose of designing a dichotomous key? How will it be utilized in this lab?
In Lab Eight, crafting a dichotomous key intends to establish a recurring instrument for labeling
an unidentified microorganism. This critical benefits as a sequential focus, leading learners via a
decision-making approach centered on distinctive attributes, allowing meticulous labels with
incredible ease.
3. Using a reputable source online, describe how the Fluid Thioglycollate Medium (FTM) test
works. Provide enough elaboration that you clearly demonstrate your knowledge of this test.
The FTM examination is accomplished to consider the oxygen conditions of microorganisms.
This is perpetrated by selecting an O2 gradient in a pipe holding thioglycollate. Microorganisms
place themselves within the tube according to their O2 prerogatives, helping aerobes,
anaerobes, and facultative anaerobes establish their diffusion along the gradient.
4. Would you consider this a selective or differential media?
differential media
5. Describe how an obligate aerobe would grow in an FTM Tube. What about a facultative
anaerobe?
It would thrive near the exterior, where O2 is plentiful. On the contrary, a facultative anaerobe
would display development throughout the pipe, with the most vigorous augmentation in the
upper layers, rich in O2, and some development expanding into the more subordinate
layers.
BIO250L”
Lab 8 Identifying an Unknown Organism BIO250L”
EXPERIMENT 1: IDENTIFICATION OF UNKNOWN BACTERIA UTILIZING A DICHOTOMOUS KEY
Introductory Questions
1. What is the number of the organism you selected in the lab?
#2
BIO250
L”
Lab 8 Identifying an Unknown Organism BIO250L”
2. Insert your dichotomous key here. Your handwritten name must be incorporated into the key,
and the key itself must have the pertinent information required to correctly identify the
microorganisms utilized in order to earn credit for this lab.
Data and Observations
Insert a photo of the gram stain you utilized in this lab, below [Show Less]