GENE 4200 practice exam 1|26 Questions with Verified Answers
Transcription factors help regulate which cell types and tissues will express a given gene
... [Show More] (T/F) - CORRECT ANSWER
When comparing RNA levels for gene X between two samples by qPCR, the sample with thehighest DCT value would have the least gene X RNA (T/F) - CORRECT ANSWER
Exons are the most abundant DNA sequences in the human genome (T/F) - CORRECT ANSWER false
Most transposons in the human genome are inactive. (T/F) - CORRECT ANSWER
Similar mechanisms are involved in promoting inheritance of DNA methylation and histone modification patterns following cell division. (T/F) - CORRECT ANSWER
A single pre-miRNA transcript typically produces miRNAs that interact with only one target mRNA. (T/F) - CORRECT ANSWER
Which of the following sequences would have the highest melting temperature when annealedto its complement?
A. AAATATATATATAAAAAAAAAAAA
B. GCGCGCGCGCGCGCGCGCGCGCGG
C. CCCCCCGGGGGGC
D. A and B will be the same
E. Insufficient information to answer the question - CORRECT ANSWER B
Protein X localizes to the nucleus under wildtype conditions. You hypothesize that coldtemperatures will cause it to localize to the cytoplasm instead. Which method would be mostappropriate for testing your hypothesis?
A. RNA in situ hybridization
B. Western blot
C. Immunohistochemistry
D. Any of the above methods will work
E. A or C will work - CORRECT ANSWER
Enhancer trap screens are used primarily to:
A. Identify regulatory regions that drive unique patterns of gene expression
B. To clarify which transcription factors bind known enhancers
C. To disrupt gene functions
D. To identify which genes are regulated by a particular enhancer
E. All of the above - CORRECT ANSWER
In an RNA seq experiment, adapters:
A. Provide universal anchors for amplification of RNA libraries for sequencing
B. Allow for bar coding of samples so many samples can be sequenced together
C. Allow RNA to be converted to cDNA
D. All of the above
E. A and B only - CORRECT ANSWER
You are interested in identifying the 10 genes that show the biggest changes in expression aftercells are treated with drug Y, when compared to untreated controls. Which method forviewing RNA-seq data from cells pre and post treatment is likely to be most informative?
A. Peak analysis
B. A heat map
C. KEGG analysis
D. A scatter plot
E. Principle component analysis - CORRECT ANSWER
Which of the following methods does not rely on hybridization of a probe?
A. Microarray
B. In situ hybridization
C. RNA-seq
D. Southern blot
E. Northern Blot - CORRECT ANSWER
Active Type 1 transposable elements:
A. Move by a cut and paste mechanism
B. Often contain promoter sequences that can impact expression of nearby genes
C. Encode for a reverse transcriptase protein
D. Move by a copy and paste mechanism
E. More than one of the above - CORRECT ANSWER
In vertebrate genomes, the modified base 5-methylcytosine is most prevalent at:
A. CpG dinucleotides
B. Long stretches of cytosines
C. CpT dinucleotides
D. Most cytosines in the genome, regardless of base context
E. A and C. - CORRECT ANSWER
If you performed a Chip Seq experiment using an antibody for HP1 you would expect to findenrichment peaks at the same places as:
A. Transcriptional start sites for active genes
B. H3K4me3
C. exons
D. H3K9me3
E. RNA polymerase 2 (polII) - CORRECT ANSWER
Concerns about off target effects from a given siRNA can be partially controlled for by:
A. Increasing the length of the siRNA
B. Raising the temperature that the experiment is performed at
C. Testing multiple siRNAs designed to different regions of the target RNA
D. All of the above
E. A and B - CORRECT ANSWER
When assessing gene expression levels, most researchers prefer qRT-PCR over Northern blots.What are two advantages of qRT-PCR over a Northern Blot? - CORRECT ANSWER
Indicate the components of the gene in the diagram below - CORRECT ANSWER
Many examples of transposons contributing to phenotypic diversity involve insertions nearpigment genes. What are two reasons that likely contribute to this? - CORRECT ANSWER
Explain why, despite being genetically identical, one of these two mice is fat and yellow whilethe other is skinny and brown. - CORRECT ANSWER
Would Xist mediated silencing of the inactive X chromosome be considered a cis or a transeffect? Why? - CORRECT ANSWER
You hypothesize that gene W is expressed in a small fraction of neurons that are dispersedthroughout the anterior portion of the mouse brain. What method discussed in class do you thinkwould be the most appropriate for testing this hypothesis and why? - CORRECT ANSWER
You perform an enhancer bashing experiment to better understand the regulatory regionsrequired for Gene X expression and get the results below. You then construct a transgene inwhich you clone only region D upstream of a minimal promoter and GFP. Do you expect thistransgene to show GFP fluorescence in the heart? What about the liver? Explain your reasoning? - CORRECT ANSWER
You are interested in identifying groups of genesthat are downregulated in response to threedifferent treatments (A, B and C) using bulk RNA-seq. *LOOK AT ELC WITH HEAT MAP* - CORRECT ANSWER
You are interested in exploring regulation of a cluster of genes in petunia leaf and stem tissues. Foreach tissue you perform RNA-seq and ChIP-seq for two different histone modifications (mod1 andmod2). You also perform a control ChIP for Histone H3 in leaf tissue. Based on the image below *LOOK AT ELC FOR PIC* - CORRECT ANSWER
You identify a new lncRNA which you hypothesize serves as decoy for the miRNA miR420. Totest this hypothesis, you examine RNA levels for several different miR420 targets in a wildtype cellline and one deleted for the lncRNA, by qRT PCR. However, you find that RNA levels for miR420target genes are comparable between the two sets of samples
a) Excluding human error, come up with a hypothesis for why you obtained this data
b) Design an experiment to test your hypothesis.
c) What would the expected outcome be if your hypothesis is correct - CORRECT ANSWER [Show Less]