Specimens that can be analyzed for carbon dioxide
1. Serum
2. Plasma
3. Whole blood
What will happen if blood is exposed to air during specimen
... [Show More] collection for pH and blood gases?
p C O 2 decreases
Henderson-Hasselbalch equation
p H = p k a + log salt / acid
What is the ratio of bicarbonate:carbonic acid in normal plasma
20:1
Lab results on a known diabetic patient revealed a decreased bicarbonate and decreased ph. Which acid base disorder would most likely cause these lab values?
Metabolic acidosis
Decrease Bicarbonate decrease p H
Normal range for pH of blood
7:35 to 7:45
Beer law concerning optical density it is:
Directly proportional to the concentration
For the ultraviolet range what part must be employed
Quartz cuvette Quartz cuvette
When performing spectrophotometer quality checks, what is the holmium oxide glass filter used to assess?
Wavelength accuracy
What is the principle of flame photometry?
Emission of a color when an element is burned
What is the principle of atomic absorption spectrophotometry?
It measures light absorption of electromagnetic radiation
What is the light source in atomic absorption instrumentation
Cathode lamp
Quenching is a disadvantage in which type of instrumentation
Fluorometry
Which analytical method measures the amount of light scattered by the particles in solution
Nephelometry
p c o 2 electrode
The actual pH of the sample has no effect
The protein at its isoelectric point
Net zero charge
In immunoelectrophoresis
There must be excess antibody present and constant trough distance between antibody and antigen
To achieve the best levels of sensitivity and specificity, a gas chromatograph may be coupled with a
Mass spectrophotometer
Which colligative property is most commonly used in osmometry
Freezing point
Zimmerman reaction is used to detect
17-ketosteroids
Hormone appears to function solely in the initiation and maintenance of lactation?
Prolactin
Hormone acts on the renal tubules to increase water reabsorption
Anti diuretic hormone
What is the major binding protein for thyroxine
Thyroid binding globulin
What is T3 uptake measuring
Thyroid binding globulin
Which hormone is important in bone and calcium metabolism
Calci tonin
Which hormone stimulates the renal production of vitamin D
PTH
Cathecholamines are secreted by
Adrenal medulla
The metabolite of epinephrine
Vanillymandelic acid
HCG is secreted by
The uterus
When does the highest level of HCG occur
First trimester
Hormone synthesized from cholesterol
Testosterone
What is the urinary metabolite so serotonin
5-hydroxyindoleacetic acid
Migration sequence from the origin, of lipoprotein during electrophoresis
Chylomicrons / beta/ pre-beta alpha
Reagents used for color development in the Liebermann-Burchard reaction for cholesterol
Acetic anhydride/sulfuric acid
The purpose of the saponification step in cholesterol methods
Convert cholesterol esters to free cholesterol
Most triglycerides procedures involve the measurement of which end product
Glycerol
Friedwald formula by which LDL cholesterol can be calculated
LDL cholesterol = cholesterol [Show Less]