BIOS390
Week 5 Exam Study Guide.Question Set 1
(TCO 6) A major difference between transcription in bacteria and eukaryotes is that bacteria such as
... [Show More] Escherichia coli have one type of RNA polymerase that is responsible for transcription of all genes. In contrast, eukaryotes have multiple nuclear DNA-dependent RNA polymerases and organelle-specific polymerases.
(TCO 6) Transcription is the process of The process by which an RNA copy of a gene is made.
(TCO 6) Transcription proceeds in the (what direction?):
Transcription always proceeds in the direction 5' (5-prime) to 3' (3-prime) on
the coding strand of DNA.
Question Set 2
(TCO 6) Define promoter:
The collection of DNA sequence elements, including the core promoter and promoter proximal elements, that are required for initiation of transcription or that increase the frequency of initiation only when positioned near the transcriptional start site.
Question Set 3
(TCO 6) Which of the following statements is correct?
A. DNA polymerase requires a primer to get started.
B. RNA polymerase requires a primer to get started.
C. Both DNA and RNA polymerase require primers to get started.
D. Both DNA and RNA polymerase can start synthesis de novo. (TCO 6) Which statement is not true about the bacterial sigma factor?
A. Sigma is a regulatory protein, which is required for the initiation of transcription.
B. Sigma has the ability to join ribonucleotides (NTPs) by phosphodiester bonds using DNA as a template.
C. Sigma properly orients the RNA polymerase complex for transcription at the gene start site.
D. After initiation is complete, sigma does not dissociate completely; some domains are displaced and mRNA synthesis continues.
(TCO 6) RNA polymerase forms a phosphodiester bond between. the 3′-OH group of the last nucleotide in the RNA chain.
Question Set 4
(TCO 6) Promoter clearance refers to a step of transcription initiation in which The process by which an RNA polymerase moves away from a promoter after initiation of transcription. [Show Less]