BIOS 390 Molecular Biology Week 7 Study Guide.Question Set 1
(TCO 10) What are minisatellites and microsatellites?
Minisatellite: A short sequence of
... [Show More] (usually) 12 bp or more repeated over and over in tandem; also known as variable number tandem repeats. Microsatellites: short tandem repeats (STRs)
Repetitive stretches of short sequences of DNA (usually 2–4 bp) used as genetic markers. A given STR is found in varying lengths scattered around a eukaryotic genome. STR analysis is currently the most widely used DNA typing procedure in forensic genetics.
(TCO 10) Repeat length for minisatellite is (usually) 12 bp (TCO 10) Repeat length for a microsatellite is (usually 2–4 bp)
Question Set 2
(TCO 10) Forensic DNA typing often involves recovery of very tiny amounts of DNA from a crime scene. What is a major concern in analyzing this DNA? Even pieces of highly degraded DNA can still be amplified, as long as the target sequence is intact. scientists must be very careful to avoid contamination with their own DNA, or other biological materials, during the identifying, collecting, and preserving of the sample.
(TCO 10) Find the incorrect statement.
1. Different individuals differ in the length of the repeat sequences.
2. For a boy child, the bands obtained during DNA typing, 50% come from dad and the remaining 50% is random.
3. Single locus probes during minisatellite analysis are more useful.
4. with the help of PCR, even a single cell is enough for DNA typing.
(TCO 10) Find the incorrect statement.
1. STR analysis is used more than minisatellite analysis.
2. The first step in STR analysis is digestion of DNA.
3. mtDNA can be used to determine maternal lineage.
4. Nuclear DNA is less stable than mtDNA.
Question Set 3
(TCO 11) Which statement is not true about cancer?
1. Cancer results from the accumulation of many genetic changes.
2. Gene mutations that increase the risk for developing cancer are always inherited.
3. Cancer cells grow in an uncontrolled and invasive way.
4. Cancer cells are immortal.
(TCO 11) The process by which cancer cells travel from the tissue of origin to other parts of the body is called metastasis [Show Less]