Biochemistry Readiness Check. Questions And Answers. Western Governors University.What is the complementary sequence to 3’ ATG CGA ATA 5’?
(1/1
... [Show More] Points)
3’ TAT TCG CAT 5’
The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’
3’ TAC GCT TAT 5’
3’ GAT AGC ATA 5’
5’ ATA AGC GTA 3’
2
Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’?
(0/1 Points)
Tyr Val Tyr
The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3') If you chose Tyr Val Tyr, this answer is incorrect because this is the amino acid sequence that results from the mRNA 5' UAC GUC UAU 3' which would have been complementary to the given coding strand. Coding DNA is non-complementary and parallel to mRNA.
Ile Gln Ile
Ile Asp Val
Leu His Lys
3
Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’?
(1/1 Points)
Tyr Tyr Arg
Ile Met Ala
Ser Val Ile
The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile
Ser His Gln
4
Which of the following are the correct components for a PCR reaction?
(1/1 Points)
dNTPs, Primer, RNA Polymerase, template RNA
dNTPs, Primer, DNA Polymerase, template DNA
The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all components are about DNA. The Primer is even a DNA Primer.
ATP, Primer, mRNA polymerase; template mRNA
Acetyl CoA, RNA primer, DNA Ligase, Template phosphate
5
Assuming 100% reaction efficiency, how many DNA copies will be produced after 5 PCR cycles, if we begin with 1 DNA template?
(1/1 Points)
16
32
The correct answer is 32. 2x2x2x2x2=32
64
10
6
Which of the following would represent a silent mutation if this is the original sequence: 5’ AUC GUA ACA 3’?
(1/1 Points)
5’ AUC GGA ACA 3’
5' AUA GUA ACA 3'
The correct answer is 5' AUC GUA ACA 3' Ile Val Thr
5’ AUG GUA ACA 3’
5’ AUC GCA ACA 3’
7
If the original coding sequence is 5’ CGA TAC TTC AGA 3’ and it is mutated to 5' CGA TAT TTC AGA 3', what type of mutation would have taken place?
(0/1 Points)
Silent
Missense
The correct answer is silent mutation. The nucleotide sequence changes, but it codes for the same amino acid. The coding sequence 5' TAC 3' corresponds to the mRNA sequence 5' UAC 3' (Tyr), and the coding sequence 5' TAT 3' corresponds to the mRNA sequence 5' UAU 3' (Tyr). Since the C changed to at T, this is a point mutation. If the point mutation results in the same amino acid in the new sequence as in the original sequence, the point mutation is a silent mutation.
Nonsense
Insertion
8
This learning objective is now tested in a different WGU course than Biochemistry. Please select True. [Show Less]