Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper
... [Show More] formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects of culturing
microorganisms onto growth media. What concepts does each experiment focus on? Why do
you think these concepts are important to the field of microbiology?
The first experiment is about preparing agar plates and putting bacteria on them. It talks about
why it's important to be really careful to keep things clean and make sure the bacteria grow
without anything else mixing in. This is super important in microbiology so we can accurately
figure out what types of bacteria we're dealing with without any extra stuff getting in the way.
The second experiment is about moving bacteria to stab tubes and agar plates. It shows how
bacteria grow differently in various conditions. This helps us learn how to grow and look at
bacteria in different types of media. These ideas are also really crucial in microbiology for
figuring out infections and studying how bacteria act.
2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools
and provide a scenario in which you would use each tool.
○ The loop is utilized for streaking microorganisms on a plate, whereas the needle is
employed for picking and transferring colonies onto agar plates.
○ Pipettes are used to take liquid samples precisely.
○ Microbial colonies from the body's tissues are picked using swabs..
○ he spreader evenly distributes bacterial colonies on a plate.
3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes.
Focus particularly on those used with E. coli used to make human insulin.
At Biotech, bioreactors are utilized to create the perfect environment for E. coli to grow.
4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to
diagnose a gastrointestinal infection. You streak your sample on a growth media containing
glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see [Show Less]