ACD/CPD/CPD2 - ANSWER-21 days
CPDA-1 - ANSWER-35 days
Infants
HDFN from ABO - ANSWER-Spherocytes
1st pregnancy
O mother
Permanent deferral
... [Show More] - ANSWER-Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER-Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER-Less than 5 x 10^6 to prevent febrile non hemolytic reactions
Cryoprecipitate - ANSWER-Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit - ANSWER-5000 10000
Irradiated blood - ANSWER-Prevent GVHD
Ulex europaeus - ANSWER-Anti H lectin
Dolichos biflorus - ANSWER-Anti A1 lectin
Amount of H greatest to least - ANSWER-O
A2
B
A1
A1B
Most immunogenicity antigen - ANSWER-D
Weak D - ANSWER-Negative immediate spin and positive AHG
Weak D donor - ANSWER-Must be labeled Rh positive
Weak D patient - ANSWER-Receives D negative
D control - ANSWER-AB positive
Any D negative
IgM antibodies - ANSWER-I
H
MN
P1
Lewis
IgG antibodies - ANSWER-Rh group
K
Duffy
Jk
I, i antibody - ANSWER-i converts to I as infant matures
Destroyed by enzymes - ANSWER-MN
S
Duffy
Enhanced by enzymes - ANSWER-Rh
Kids
Lewis
I
P1
Dosage - ANSWER-MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality - ANSWER-TRALI, hemolytic transfusion reactions and TACO
IgE antibodies - ANSWER-Mild Allergic reactions
IgA antibodies - ANSWER-Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT - ANSWER-Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
Kernicterus - ANSWER-Excess bilirubin in newborn
HDFN from Rh - ANSWER-Retics
DAT positive
Immediate jaundice
After first baby
Rosette - ANSWER-Screening for fetal hemorrhage
Kleihauer Betke - ANSWER-Quantitative fetal maternal bleed
Primary immune response - ANSWER-IgM
Secondary immune response - ANSWER-IgG
Type 1 hypersensitivity - ANSWER-Anaphylactic
Immediate
Type 2 hypersensitivity - ANSWER-Antibody depending cytotoxicity
Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER-Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER-Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER-Antibodies added to pre-cut wells in center of agar plate and patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER-Sandwich technique
HCG
Nephelometry - ANSWER-Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER-Add florescence labeled anti-body to patient tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER-Add patient serum to reagent wash add florescence label to anti-globulin wash and examined under microscope
FPIA (Fluorescence Polarization Immunoassay) - ANSWER-Add reagent antibody and fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER-TP/ TP + FN x 100
Specificity - ANSWER-TN / TN + FP x 100
Non lattice - ANSWER-More sensitive immunoassays nephelometry
Lattice - ANSWER-Less sensitive
C reactive protein - ANSWER-Acute phase protein
Inflammation
Syphilis - ANSWER-T palladium
FTA abs
TPI
Dark field microscopy
VDRL - ANSWER-Syphilis CSF screening but can be positive for malaria
RPR - ANSWER-Charcoal for syphilis
More sensitive but Les specific
infectious mononucleosis - ANSWER-EBV
Lymphocytes
B cells
Burkets disease?
Autoimmune diseases - ANSWER-SLE
Sjögren's syndrome
Scleroderma
RA
Recent acute hepatitis A infection - ANSWER-Anti HaV
Acute hep B infection - ANSWER-Highly infectious
HBsAg
HBeAg
Chronic hep B - ANSWER-Carrier
Anti HBc
Past infection immunity to hep B - ANSWER-Anti HBe
Anti HBc
Anti HBs
Vaccine immunity to hep B - ANSWER-Anti HBs
Hep c infection - ANSWER-Anti HcV
Graves' disease - ANSWER-Hyperthyroidism
Low TSH
Increase production T3 and T4
Weight loss and anxiety
Hadimotos - ANSWER-Hypothyroidism
Increase TSH
Weight gain lethargy intolerance to cold
Thyroglobulins
rheumatoid arthritis - ANSWER-IgG Fc
RA factor
CA 125 - ANSWER-Ovarian cancer
CA 19-9 - ANSWER-Pancreases cancer
CEA - ANSWER-Colon breast lung cancer
CA 15-1 - ANSWER-Breast cancer
Transferrin - ANSWER-Iron transport
Ferritin - ANSWER-Iron storage
Hemoglobin F - ANSWER-Alpha and gamma globlin chains
Hemoglobin a - ANSWER-Alpha and beta globin chains
Left shift - ANSWER-Decreased CO2,
increased pH with high affinity and
decreased 23DPG
Right shift - ANSWER-Increased CO2
Decreased pH
Increased two, three DPG
Hemoglobin Reference range - ANSWER-Males 14-18
Females 12-16
Hematocrit reference ranges - ANSWER-Males is 42% to53%
Females are 38% of 47%
MCV - ANSWER-800-100
Hct/rbc x 10
MCH - ANSWER-28-32 pg
Hgb/rbc x 10
MCHC - ANSWER-32-36%
Hgb/ hct x 100
Rule of 3 - ANSWER-Hgb x3 = hct
RDW - ANSWER-11-14%
WBC reference range - ANSWER-5000-10000
Neutrophils reference range - ANSWER-Relative 45-70%
Bacteria
RBC RR - ANSWER-Males 4.5-6.1 x 10^6
Females 3.8-5.2 x 10^6
Lymphocyte RR - ANSWER-Relative 20-40%
Viral infection
Monocyte - ANSWER-Relative 3-10%
Basophils - ANSWER-Relative 0-2%
Inflammation response mediator
Eosinophils - ANSWER-Relative 0-3%
Allergic response
Hyper-segmented neutrophils - ANSWER-Megaloblastic anemia
B12 and folate deficiency
BAnd neutrophils (hypo-segmented) - ANSWER-PelegerHuey
Aml
Aids
Uncleared red cell WBC correction - ANSWER-Wbc X 100 / 100+NRBC
Dohle bodies - ANSWER-May hagglin
Hazy blue inclusion on NEUTROPHILS
May hagglin - ANSWER-Dohle bodies
Thrombocytopenia with stress platelets
Myeloproliferation
Chediak higashi disease - ANSWER-Giant lysosomal granules in granulocytes monocytes and lymphocytes
Infections and bleeding
Alder-Reilly anomaly i - ANSWER-Darks large metachromatic cytoplasm is granules
Anisocytosis - ANSWER-Variation in size when RDW is greater than 14.5
Polychromatsia - ANSWER-Blue haze in RBC is stained with new methylene blue
Seen with retics
poikilocytosis - ANSWER-Variation in shape
Sever anemia
Acanthocytes - ANSWER-Spur cell
Starfish
Sever liver disease
McLead syndrome
Echinocyte - ANSWER-Burr cell
Small projections all around
Uremia
Pyruvate kinase deficiency
Elliptocyte - ANSWER-HE
Iron deficiency
Thalassemia
Macro-ovalocyte - ANSWER-Megaloblastic anemia
Helmet cell - ANSWER-Keratocyte
RBS fragment
Hemolytic anemia and burns
Shistocytes - ANSWER-RBC fragments
DIC
Hemolytic anemia
Spherocytes - ANSWER-HS
Increase MCHC
ABO HDN
Immune hemolytic anemia
Stomatocyte - ANSWER-Mouth in the middle of RBC or slit
H stomatocytosis
Liver disease (acquired)
Target cells - ANSWER-Liver disease
Hgb c
Thalassemia
Pericious anemia
Tear drop cells - ANSWER-Dacryocytes
Thalassemia
Megaloblastic anemia
PA
Hgb c crystal - ANSWER-Hexagonal dense hgb with RBC membrane
Hgb sc crystal - ANSWER-Finger like or quartz like dense hemoglobin protruding from the RBC membrane
Howell jolly body - ANSWER-DNA just one on RBC
Post removal of spleen
Megaloblastic anemia
Hemolytic anemia
Basophilic stippling - ANSWER-lead poisoning
Thalassemia
RNA
Pappenheimer bodies - ANSWER-Iron
Small clusters around RBC
Prussian blue
Sideroblastic anemia
Heinz bodies - ANSWER-denatured hemoglobin
Small dots on RBC not clusters
G6PD deficiency
Cabot rings - ANSWER-Figure 8
Mitotoic spindle
Megaloblastic anemia
Increase MCV
Increase MCHC (37)
Decrease RBC - ANSWER-Cold agglutinate
Rule of 3 not fitting consider - ANSWER-Clots
Cold agglutination
Lipemic icteric hemolyzed
Poikilocytosis
Increase ESR - ANSWER-Greater than 20
Increase plasma proteins
Tilting tube
Increase temp
Standing to long
Excess EDTA
Decrease ESR - ANSWER-QNS
Decrease temp
Retics - ANSWER-Seen with super vital stain
Increase in hemolytic anemia blood loss and therapy
Absolute retics - ANSWER-%retics x RbC
% retics - ANSWER-Retics in 100 RBC / 10
Correction retics - ANSWER-Retic % x hct / 45
Non megaloblastic - ANSWER-Macrocyclic
Liver disease
Target cells
Megaloblastic - ANSWER-B12 deficiency
Folate deficiency
Marcrocytic
PA - ANSWER-B 12 deficiency
Howel Holley bodies
Macrocyclic
Microcytic because of heme - ANSWER-Iron deficiency
Sideroblastic
I disease of inflammation [Show Less]