ASCP MB EXAM QUESTIONS AND CORRECT ANSWERS RATED A+/ ASCP MB EXAM LATEST 2023/2024/ASCP MB EXAM 2023 NEW UPDATE WITH
SOLUTIONS 100% CORRECT/ASCP REAL
... [Show More] EXAM
(RATED A+)
RNA Polymerase I - ANSWERS-DNA template
Location: Nucleous
Products: 18s, 5.8s, 28s rRNA
RNA Polymerase II - ANSWERS-DNA template
Location: Nucleus
Products: mRNA, snRNA
RNA Polymerase III - ANSWERS-DNA template
Location: Nucleus
Product: tRNA, 5s rRNA, snRNA
Mitochondrial RNA Polymerase - ANSWERS-DNA template, product: mRNA
Mammalian DNA polymerase α - ANSWERS-DNA template, product: primers
HCV RNA Polymerase - ANSWERS-RNA template, product: viral genome
Dengue virus RNA Polymerase - ANSWERS-RNA template, product: viral genome
Poly A Polymerase - ANSWERS-No template, product: Poly A tail
230 wavelength - ANSWERS-Contamination of organic compounds
270 wavelength - ANSWERS-Phenol contamination
280 wavelength - ANSWERS-Protein contamination
>330 wavelength - ANSWERS-Particulate matter contamination
Southern Blot - ANSWERS-DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis and ID, gene structure
Northern Blot - ANSWERS-RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression
Western Blot - ANSWERS-Protein Target
Protein probe
Purpose: Protein processing, gene expression
Southwestern Blot - ANSWERS-Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation
Eastern blot - ANSWERS-Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein processing, gene expression), detection of specific agriculturally important proteins
Far-eastern blot - ANSWERS-Lipid target
No probe
Purpose: Transfer separated lipids to membranes for analysis by mass spectometry
PCR Primers - ANSWERS-Direct DNA synthesis to desired region
PCR: dATP, dCTP, dGTP, dTTP - ANSWERS-Building blocks that extend primers
PCR KCl - ANSWERS-Salt for optimal hybridization of primers to template
PCR: Tris buffer - ANSWERS-Buffer to maintain optimal pH for enzyme reaction
PCR: MgCl2 - ANSWERS-Divalent cation, required by enzyme
PCR Polymerase - ANSWERS-Polymerase enzyme that extends the primers, adds the dNTPs
PCR: copies of template - ANSWERS-Sample DNA that is being tested
Denaturation temps/time - ANSWERS-90-96, 20-60 sec
Annealing temps/time - ANSWERS-50-70, 20-90 sec
Extension temps/time - ANSWERS-68-75/10-60 sec [Show Less]