6-1 The process of DNA replication requires that each of
the parental DNA strands be
used as a
... [Show More] to produce a duplicate
of the opposing strand.
(a) catalyst
(b) competitor (c)
template
(d) copy
6-2 DNA replication is considered semiconservative because
.
(a) after many rounds of DNA replication, the original DNA double helix is still
intact.
(b) each daughter DNA molecule consists of two new strands copied from the
parent DNA molecule.
(c) each daughter DNA molecule consists of one strand from
the parent DNA
molecule and one new strand.
(d) new DNA strands must be copied from a DNA template.
6-3 The classic experiments conducted by Meselson and Stahl
demonstrated that DNA
replication is accomplished by employing a mechanism.
(a) continuous
(b) semiconservative
(c) dispersive
(d) conservative
6-4 Initiator proteins bind to replication origins and
disrupt hydrogen bonds between
the two DNA strands being copied. Which of the factors
below does not contribute to
the relative ease of strand separation by initiator proteins?
(a) replication origins are rich in A-T base pairs
(b) the reaction can occur at room temperature
(c) they only separate a few base pairs at a time
(d) once opened, other proteins of the DNA replication
machinery bind to the
origin
6-5 If the genome of the bacterium E. coli requires
about 20 minutes to replicate itself,
how can replicated
(a) The Drosophila genome is smaller than the E. coli
genome.
(b) Eukaryotic DNA polymerase synthesizes DNA at a much faster
rate than
prokaryotic DNA polymerase.
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(c) The nuclear membrane keeps the Drosophila DNA concentrated in
one place
in the cell, which increases the rate of polymerization.
(d) Drosophila DNA contains more origins of replication
than E. coli DNA.
Meselson and Stahl grew cells in media that contained different
isotopes of nitrogen (15N and 14N) so that the DNA molecules
produced from these different isotopes could be distinguished by mass.
A. Explain how “light” DNA was separated from “heavy”
DNA in the Meselson
and Stahl experiments.
6-
6
were begun, and explain how one of them was ruled out
definitively by the
experiment you described for part A.
C. What experimental result eliminated the dispersive model of DNA
replication?
6- Indicate whether the following statements are true or false. If
7 a statement is false, explain why it is false. [Show Less]