Biochemistry Readiness Check
The 2nd OA Readiness Check is designed to give you additional practice with new assessment-style questions to help prepare fo... [Show More] r success on the OA. Thus, please try to mimic exam-like conditions by taking the test in one <2 hr sitting without any study aids apart from your whiteboard/scrap paper. Note: Responses cannot be edited. You will be able to see the results and feedback after submitting the test. A score of 70% is required for OA approval. If you score below 70%, please speak with a Course Instructor via appointment to discuss next steps prior to retaking this test.
What is the complementary sequence to 3’ ATG CGA ATA 5’?
3’ TAT TCG CAT 5’
The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’
3’ TAC GCT TAT 5’
3’ GAT AGC ATA 5’
5’ ATA AGC GTA 3’
Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’?
Tyr Val Tyr
The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3') If you chose Tyr Val Tyr, this answer is incorrect because this is the amino acid sequence that results from the mRNA 5' UAC GUC UAU 3' which would have been complementary to the given coding strand. Coding DNA is non-complementary and parallel to mRNA.
Ile Gln Ile
Ile Asp Val
Leu His Lys
Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’?
Tyr Tyr Arg
Ile Met Ala
Ser Val Ile
The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile
Ser His Gln
Which of the following are the correct components for a PCR reaction?
dNTPs, Primer, RNA Polymerase, template RNA
dNTPs, Primer, DNA Polymerase, template DNA
The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all components are about DNA. The Primer is even a DNA Primer.
ATP, Primer, mRNA polymerase; template mRNA
Acetyl CoA, RNA primer, DNA Ligase, Template phosphate [Show Less]